Competence center for microarray-based comparative genomic hybridisation (Array-CGH)

The method of microarray-based comparative genomic hybridisation (Array-CGH or Matrix-CGH) was developed in 1997 by Solinas-Toldo et al. in the DKFZ in Heidelberg. Array-CGH is an advancement of the comparative genomic hybridisation (conventional CGH) and allows the proof of profit and loss in the genomic DNA. A comparative genomic hybridisation is made against a grid of immobilized DNA fragments (matrix or DNA chip) and the relation between the fluorescence signals of the patient's DNA and a reference DNA is determined (see figure). In our institute Array-CGH is used as a supplement for the spectrum of classical cytogenetic methods in the areas of postnatal cytogenetics, (prenatal cytogenetics) and tumour cytogenetics.

Our team concentrates on the identification and characterisation of chromosomal aberrations in patients with innate malformations, in particular skeletal malformations, and/or mental retardation without pathological findings of chromosome analyses. The use of high resolution molecular-cytogenetic methods such as Array-CGH makes a genomewide detection of submicroscopic chromosome changes such as microdeletion or microduplications and an exact identification of chromosomal breakpoints, e.g. at unbalanced structural aberrations, possible.
Different array-platforms for the analyses of DNA of patients are used in our team: BAC-arrays and oligonucleotide-arrays (e.g. Agilent 244K).

Principle of the Array-CGH-method

Contact

Dr. med. Dirk Korinth

Scientist

CharitéUniversitätsmedizin Berlin

CVK: Campus Virchow-Klinikum

Charité Center Gynecology, Perinatal, Pediatric & Adolescent Medicine with Perinatal Center & Human Genetics CC 17

Institute of Medical Genetics and Human Genetics

Institute of Medical Genetics and Human Genetics

Postal address:

Augustenburger Platz 1

13353 Berlin

Internal address:

Südring 11

t: +49 30 450 569 141

f: +49 30 450 566933

Map

Contact

Dr. med. Dirk Korinth
Scientist
t: +49 30 450 569 141